An Open Source Technology Platform to Manufacture Hydrogel-Based 3D Culture Models in an Automated and Standardized Fashion

Current mixing steps of viscous materials rely on repetitive and time-consuming tasks which are performed mainly manually in a low throughput mode. These issues represent drawbacks workflows that can ultimately result irreproducibility research findings. Manual-based further limiting the advancement widespread adoption materials, such as hydrogels used for biomedical applications. challenges be overcome by using automated with standardized processes to increase reproducibility. In this study, we present step-by-step instructions use an open source protocol designer, operate workstation, identify reproducible mixtures. Specifically, designer guides user through experimental parameter selection generates ready-to-use code workstation. This workstation is optimized pipetting enable highly reliable handling integration temperature docks thermoresponsive positive displacement pipettes optional tip touch dock remove excess material from pipette tip. The validation verification mixtures fast inexpensive absorbance measurement Orange G. presents results obtain 80% (v/v) glycerol mixtures, dilution series gelatin methacryloyl (GelMA), double network 5% (w/v) GelMA 2% alginate. A troubleshooting guide included support users adoption. described workflow broadly applied number generate user-defined concentrations fashion.